Paradiso

This research focuses on improving the surgical removal of the Internal Limiting Membrane ILM, a thin layer in the retina vital for vision, especially in patients with macular edema. The study evaluates the properties and safety of different dyes used during eye surgery to make ILM removal safer and easier. The commonly used dye Indocyanine Green ICG stains well but may have toxic effects, while alternatives like Brilliant Blue G BBG are safer but less popular. Researchers aim to find or modify dyes for better staining and safer use during surgery. The study observes the use of vital dyes, including a new Heavy BBG BBG with Deuterium Oxide and the impact of intraoperative light filters like orange, green, and yellow filters during vitrectomy with xenon endoillumination. It examines how these dyes and filters affect the visibility and handling of the ILM. New dye preparations and protocols are tested to improve staining and grip without increasing toxicity. The study involves no additional patient treatment beyond standard surgery. Participants undergo vitrectomy using one of the three dyes BBG, ICG, or Trypan Blue combined with sequential use of light filters to determine the best contrast. Researchers analyze removed ILM tissue samples for thickness, stiffness, and surface features, as well as video recordings to assess dye contrast. No follow-up patient contact is required after surgery, and analyses may take up to a year. The study focuses on careful measurement of tissue properties and dye effects to guide safer and more effective surgical techniques.