Characterizing Monocyte Types in Adults Critically Ill With Pseudomonas Aeruginosa Sepsis

What this Trial Is About

This research aims to understand the immune response in critically ill patients with sepsis caused by Pseudomonas aeruginosa PSA. It focuses on whether the pro-inflammatory M1 or anti-inflammatory M2 type of monocytes dominate in the blood and how this relates to the severity of the sepsis and the patients outcome. The study builds on previous findings that anti-inflammatory monocytes may be more common in sepsis but seeks to explore this further specifically for PSA infections. The study collects blood samples from critically ill patients in intensive care units who have confirmed PSA infections. Researchers analyze monocytes by measuring specific surface markers using flow cytometry and assess cytokine patterns both in the patients and after stimulating the cells outside the body with lipopolysaccharide LPS. This helps to characterize the types and functions of monocytes present during PSA sepsis. Participants have their blood drawn to isolate immune cells for testing and comparison with healthy controls. Researchers monitor monocyte surface markers and cytokine levels over time. The main outcome is measuring monocyte surface marker expression over two years, while secondary outcomes include cytokine concentration changes after stimulation over four years. This observational study helps to clarify immune cell behavior during PSA sepsis and may guide future treatments.

Monocyte Profiles in Critically Ill Patients With Pseudomonas Aeruginosa Sepsis