Actively Recruiting
Nanopore Sequencing in Ophthalmology - a Paradigm Shift in Pathogen Determination?
Led by Medical University of Graz · Updated on 2025-09-30
20
Participants Needed
1
Research Sites
52 weeks
Total Duration
On this page
AI-Summary
What this Trial Is About
Researchers are evaluating the use of the Oxford Nanopore MinION device to identify pathogens in eye samples from patients with endophthalmitis, an infection inside the eye. This pilot study aims to assess the feasibility of using this low-cost DNA/RNA sequencing tool compared to standard microbial culture methods. The study is observational and seeks to improve pathogen detection in ophthalmology. During routine vitrectomy surgery, fluid samples from the eye are collected and split for two tests: standard culture to grow microbes and sequencing using the MinION device. The sequencing results are compared to culture results to identify pathogens. Clinical data including visual function before and after surgery are also collected. Treatment decisions are not changed based on sequencing results. Participants will have samples taken during their vitrectomy procedure. Researchers will analyze the samples using both methods, with sequencing results available in about 10 hours and culture results taking up to 14 days. Visual acuity will be gathered from patient records two months after surgery. The study monitors pathogen detection timing and visual outcomes without impacting patient treatment. Participation lasts through surgery and follow-up visits.
CONDITIONS
Brief Title
Nanopore Sequencing in Ophthalmology
Who Can Participate
Eligibility Criteria
You may qualify if you...
- Eyes of patients with endophthalmitis
You will not qualify if you...
- No informed consent possible
- Participation in other studies
AI-Screening
AI-Powered Screening
Complete this quick 3-step screening to check your eligibility
Your Study Journey
Duration - 2 to 4 weeks
Participants are screened for eligibility to participate in the trial.
1 visit (in-person)
Duration - Day of vitrectomy
Participants undergo routine vitrectomy during which vitreous samples and anterior chamber taps are collected for pathogen detection using both nanopore sequencing and microbiological culture.
1 visit (in-person during surgery)
Duration - Up to 2 months after vitrectomy
Clinical features and visual function are monitored from patient charts before and up to two months after vitrectomy without altering treatment based on sequencing results.
Follow-up assessments via patient chart review
Trial Site Locations
Total: 1 location
1
Universitäts Augenklinik Graz
Graz, Styria, Austria, 8036
Actively Recruiting
Research Team
M
Michael Sommer, MD
G
Gerald Seidel, MD, PhD
How is the study designed?
Study Type
OBSERVATIONAL
Masking
N/A
Allocation
N/A
Model
N/A
Primary Purpose
N/A
Number of Arms
0
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