Actively Recruiting

Age: 0 - 1Hour
All Genders
Healthy Volunteers
NCT00747851

NETs: Protection or Harm in Neonatal Inflammation or Infection

Led by University of Utah · Updated on 2025-12-05

388

Participants Needed

1

Research Sites

1178 weeks

Total Duration

On this page

Sponsors

U

University of Utah

Lead Sponsor

E

Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD)

Collaborating Sponsor

AI-Summary

What this Trial Is About

This is a prospective in vitro cell biology study of polymorphonuclear leukocyte (PMN) protein synthesis in response to PAF. PMNs from cord blood of premature human infants at risk for NEC (birth weight between 501 - 1500 grams) and PMNs from cord blood of healthy term infants will be isolated and stimulated with PAF, a biologically active phospholipid implicated in the pathogenesis of NEC. NEC, a disease of prematurity with an incidence of 10.1% of infants born weighing between 501 - 1500 grams, is associated with significant morbidity and mortality. We will compare the protein synthesis of inflammatory modulators, including Interleukin 6 Receptor alpha (IL-6R alpha) and Retinoic Acid Receptor alpha (RAR alpha) proteins to protein synthesis responses already observed in PMNs isolated from healthy adults. Furthermore, we will characterize the expression and activity of the mammalian target of rapamycin (mTOR) translational protein synthesis control pathway in PMNs isolated from preterm and term infants and compare those results with previous observations in PMNs isolated from adults. This pathway is known to regulate IL-6R alpha and RAR alpha protein expression in PMNs isolated from adults. We will also follow those premature infants at risk for NEC clinically to determine which infants develop NEC and what risk factors may be associated with NEC in this population.

CONDITIONS

Official Title

NETs: Protection or Harm in Neonatal Inflammation or Infection

Who Can Participate

Age: 0 - 1Hour
All Genders
Healthy Volunteers

Eligibility Criteria

Eligible

You may qualify if you...

  • Patients hospitalized in the NICU who were less than or equal to 1500 grams or less than 30 weeks gestational age at birth
  • Term infants delivered at UUMC without complication, either via cesarean section or vaginal delivery
  • Cord blood isolated within first hour of life
  • Parents or guardians must have signed informed consent
Not Eligible

You will not qualify if you...

  • Infants with major congenital anomalies will be excluded

AI-Screening

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Trial Site Locations

Total: 1 location

1

University of Utah

Salt Lake City, Utah, United States, 84112

Actively Recruiting

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Research Team

C

Christian C Yost, M.D.

CONTACT

How is the study designed?

Study Type

OBSERVATIONAL

Masking

N/A

Allocation

N/A

Model

N/A

Primary Purpose

N/A

Number of Arms

0

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NETs: Protection or Harm in Neonatal Inflammation or Infection | DecenTrialz