Non-invasive Diagnostic Approach for Tracking Fibrotic Changes in Myeloproliferative Neoplasms Using CCR2 Detection with Blood Tests and PETCT Imaging

What this Trial Is About

Researchers are studying chronic Philadelphia-negative myeloproliferative syndromes, which are blood disorders including essential thrombocythemia, polycythemia vera, and myelofibrosis. Myelofibrosis may occur initially or develop from the other conditions and is marked by scar-like tissue in the bone marrow, indicating a more severe disease stage. Currently, bone marrow biopsy is the only way to assess this fibrosis, and the study aims to find less invasive methods by tracking cells expressing the CCR2 receptor, a marker of fibrosis. The study evaluates a new diagnostic approach using flow cytometry on blood samples to identify CCR2-positive cells and PET-CT imaging with a targeted tracer called 68Ga-DOTA-ECL1i that binds to these cells. This approach is being tested both in preclinical models and in patients with myeloproliferative neoplasms. The project includes tracking these cells for diagnosis, functional imaging in mice, and imaging in human participants to support diagnosis and monitoring. Participants will have their blood tested for CCR2 expression on certain cells and undergo PET-CT imaging sessions using the tracer. Researchers will measure the percentage of circulating CD34CCR2 cells as the main outcome and assess bone marrow activity and tracer uptake in other sites during imaging. The study supports diagnosis and disease monitoring without invasive biopsies and includes follow-up assessments to understand fibrotic progression. Participation involves blood tests and imaging, with evaluations done at enrollment and imaging sessions.

A New Diagnostic Algorithm to Non-invasively Track Fibrotic Changes in Myeloproliferative Neoplasms Based on C-C Chemokine Receptor 2 Detection. From Flow Cytometry to the Development of Targeted Positron Emission Tomography Molecular Imaging. Pre-clinical Studies and First In-human Proof of Concept