Evaluation of a Rapid Diagnostic Tool MinION to Identify Microorganisms in Foot Wounds of Patients with Diabetic Foot Osteomyelitis

What this Trial Is About

Diabetic foot wound infections often involve multiple types of bacteria, but traditional culture methods may not detect all bacteria involved and take time, which can delay appropriate antibiotic treatment. Researchers are studying a new rapid test using Oxford Nanopore Technologies MinION device, which can identify nearly all microbial genomes and antibiotic resistance in a sample within four hours. This study aims to compare the diversity of microbes found in bone biopsies from diabetic foot osteomyelitis patients using the MinION method versus conventional culture techniques. The MinION device sequences bacterial or fungal genomes quickly, providing detailed information about the microorganisms present and their potential resistance to antibiotics. Bone samples from patients with diabetic foot osteomyelitis will be analyzed using both the MinION method and standard lab cultures MALDI-TOF. Researchers will study the variety of microbes detected, their antibiotic sensitivity profiles, and any new potentially harmful species related to wound progression over one year. Participants will have bone biopsy samples taken to assess the microorganisms present in their infected foot wounds. The study will measure and compare the number and types of bacterial and fungal species found by the new MinION technology and by conventional culture methods. Additional outcomes include tracking antibiotic resistance, wound progression at multiple time points, and cost analysis of both diagnostic approaches. The study duration is planned for 12 months, with ongoing monitoring of the microbiota and infection characteristics.

New Diagnostic Tool (MinION) for Identifying Microorganisms in Foot Wounds of Patients Living With Diabetic Foot Osteomyelitis