Study of Immune and Inflammatory Responses in Bone Damage During Periodontitis and Peri-Implantitis with Biological Sample Collection

What this Trial Is About

Researchers are studying the immune and inflammatory responses involved in bone destruction during periodontitis and peri-implantitis. The goal is to identify specific molecular markers that reflect disease activity, improve understanding of disease mechanisms, and find potential therapeutic targets. This observational study collects biological samples from patients with stage 3 or 4 periodontitis or peri-implantitis, as well as from patients with healthy gums requiring surgical care, to compare molecular profiles and immune responses. Participants are grouped into three categories those with stage 3 or 4 periodontitis, those with peri-implantitis affecting at least one implant, and controls with healthy gums needing surgery for other dental reasons. Biological samples such as plaque, saliva, gingival fluid, and gingival tissue during surgery are collected. Cases receive periodontal or peri-implantitis treatment including non-surgical care, possible surgery if needed, and follow-up evaluations. Controls undergo surgical procedures like dental avulsion or pre-prosthetic surgery with sample collection and healing monitoring. Throughout the study, clinical data and biological samples will be collected at inclusion and multiple follow-up points to analyze inflammatory mediators, cytokines, proteases, and bacteria. Researchers will use techniques like Luminex and quantitative PCR to characterize immune markers and microbial profiles. The primary outcome is the analysis of cytokines in gingival fluid. Secondary outcomes include cytokine expression in cells, protease characterization, and microbiological biofilm analysis. The study follows patients as part of their routine care without additional follow-up, lasting up to 60 months for recruitment and sample collection.

Periodontitis and Inflammation