Study on Neutrophil Roles and Immune Response in Periodontitis Using Biological Samples

What this Trial Is About

Periodontitis is a widespread chronic inflammatory disease in adults that causes permanent damage to the tissues supporting the teeth. It is linked to oral bacteria like Porphyromonas gingivalis and involves an abnormal immune response. This study aims to understand the role of different neutrophil subtypes, a type of immune cell, in the progression from gingivitis to periodontitis and their involvement in tissue destruction. Researchers seek to identify specific neutrophil interactions with the oral environment and bacteria to better detect patients at risk for periodontitis. The study is an observational, cross-sectional case-control design conducted at a single center. It includes three groups of adult patients requiring dental surgery those with periodontitis, gingivitis, or healthy gums. Participants will provide biological samples such as tissue, saliva, blood, and gingival fluid during routine surgical care. Sampling includes tooth extraction with gingival tissue collection and follow-up visits to monitor healing. The study collects data over a 36-month inclusion period without additional follow-up specific to the research. Participants will have various samples collected and analyzed to identify neutrophil subtypes and their interactions with tissue proteins and oral bacteria through advanced imaging techniques. Researchers will assess neutrophil function and their role in bone resorption related to periodontitis. The primary outcome measures focus on neutrophil subtype characterization, while secondary outcomes evaluate neutrophil interactions and activity. The total participation is aligned with usual patient care timelines and includes healing assessments after tissue sampling.

Periodontitis and Inflammation: Study on Biological Samples