Studying Blood Biomarkers During Exercise to Understand Insulin Resistance in People with Kidney Failure on Dialysis and Those with Metabolic Syndrome

What this Trial Is About

Researchers are evaluating muscle metabolism and insulin resistance during exercise in three groups patients with chronic kidney disease on dialysis, patients with metabolic syndrome, and healthy individuals aged 40 to 75. The study aims to identify a biological and functional signature of muscle-related insulin resistance by analyzing plasma metabolic intermediates during exercise. This understanding could improve assessment methods and guide muscle rehabilitation protocols, especially for dialysis patients where current insulin resistance tests are limited. Participants will undergo a metabolic stress test using a cycloergometer with gas exchange measurements taken every 6 minutes at rest and during increasing exercise intensities up to 60% of their estimated maximum power. Blood samples will be collected via a catheter at rest and after each exercise stage to measure plasma metabolites. Body composition will also be analyzed through impedancemetry. The study compares metabolic profiles adjusted for oxygen consumption across groups at multiple time points during exercise. During the study, participants will perform the exercise test lasting around 45 minutes with blood samples taken at baseline and at 6-minute intervals up to 30 minutes. Researchers will assess plasma lactate and other metabolic markers related to energy pathways and insulin resistance. Anthropometric measurements like weight, height, and waist circumference will be recorded. The primary outcome is the comparison of oxygen-adjusted plasma lactate profiles at rest and during exercise among the three groups. Secondary outcomes include comparisons of other metabolic intermediates and body composition. The study duration per participant is confined to the testing session with no long-term follow-up mentioned.

Plasma Biomarkers of Muscle Metabolism During Exercise to the Assessment of Insulin Resistance in CKD Dialysis Patients