Actively Recruiting

Age: 18Years +
All Genders
ID06467864

Predicting Ventilator-associated Lower Respiratory Tract Infection Outcomes Using Sequenced-based Early Microbiological Response: A Multi-center Prospective Study

Led by The First Affiliated Hospital with Nanjing Medical University · Updated on 2025-05-23

200

Participants Needed

1

Research Sites

4 weeks

Total Duration

On this page

Sponsors

T

The First Affiliated Hospital with Nanjing Medical University

Lead Sponsor

S

Shanghai East Hospital of Tongji University

Collaborating Sponsor

AI-Summary

What this Trial Is About

Researchers are studying ventilator-associated lower respiratory tract infections, focusing on how changes in local pathogen levels relate to patient outcomes. They are evaluating a tool called QtNGS that measures these pathogens using advanced sequencing techniques. This multi-center prospective study aims to better understand the connection between pathogen abundance before and after treatment and how it affects recovery or complications. Participants will have bronchoscopy procedures to collect lung fluid samples at enrollment and three days after treatment. These samples will help measure pathogen levels using the QtNGS tool. The study does not involve experimental treatments but observes patients who have been on mechanical ventilation and have signs of infection. During the study, participants will undergo bronchoscopy at two time points to collect fluid for analysis. Researchers will track clinical outcomes including mortality within 28 days after enrollment. The study involves careful monitoring of infections by specific bacteria and collects data to assess the relationship between early microbiological changes and patient prognosis over about one month.

CONDITIONS

Brief Title

Predicting Ventilator-associated Lower Respiratory Tract Infection Outcomes Using Sequenced-based Early Microbiological Response

Who Can Participate

Age: 18Years +
All Genders

Eligibility Criteria

Eligible

You may qualify if you...

  • Aged 18 years and above
  • Previously relied on mechanical ventilation (endotracheal intubation or tracheotomy) for breathing assistance for more than 48 hours
  • Presence of lower respiratory tract infection based on at least two of the following: abnormal temperature (greater than 38.5�B0C or less than 36.5�B0C), abnormal white blood cell count (greater than 12 x 10^9/L or less than 4 x 10^9/L), and purulent tracheal secretions
Not Eligible

You will not qualify if you...

  • Bronchoscopy and respiratory specimen collection not performed at screening (Day 1) and after 3 days of treatment (Day 4)
  • Patient or family refusal to participate in the study
  • No evidence of infection by study-associated pathogens based on bacterial culture from bronchoalveolar lavage fluid at initial screening
  • Infection must be with one of the following single pathogens: Pseudomonas aeruginosa, Acinetobacter baumannii, Klebsiella pneumoniae, or Staphylococcus aureus; patients with other infections are excluded
  • Evidence of infection defined as single positive bacterial culture with pathogen quantification 210^4 cfu/ml or "++" and above on Day 1

AI-Screening

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Your Study Journey

Screening

Duration - 2 to 4 weeks

Participants are screened for eligibility to participate in the trial.

1 visit (in-person) including bronchoscopy and respiratory specimen collection

Diagnostic Evaluation

Duration - 4 days

Participants undergo bronchoscopy and bronchoalveolar lavage fluid collection at enrollment and three days after treatment to assess infection status.

2 visits (in-person) on Day 1 and Day 4

Long-term Monitoring

Duration - 28 days

Participants are observed for outcomes including 28-day mortality following enrollment.

Follow-up visits as per routine clinical care

Trial Site Locations

Total: 1 location

1

he First Affiliated Hospital of Nanjing Medical University

Nanjing, Jiangsu, China, 21000

Actively Recruiting

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Research Team

W

Wenkui Sun, Dr

J

Ji Zhou, Dr

How is the study designed?

Study Type

OBSERVATIONAL

Masking

N/A

Allocation

N/A

Model

N/A

Primary Purpose

N/A

Number of Arms

0

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