Actively Recruiting

Phase Not Applicable
Age: 20Years - 45Years
MALE
ID07345455

Effect of Probiotics on Sperm Quality in Male Infertility Patients

Led by Mackay Memorial Hospital · Updated on 2026-01-15

60

Participants Needed

1

Research Sites

N/A

Total Duration

On this page

Sponsors

M

Mackay Memorial Hospital

Lead Sponsor

G

Grape King Bio Ltd.

Collaborating Sponsor

AI-Summary

What this Trial Is About

Researchers are evaluating the impact of probiotic supplements on sperm quality in men diagnosed with infertility, focusing on unexplained oligozoospermia, asthenozoospermia, or oligoasthenoteratozoospermia. Male infertility, which accounts for about 40% of infertility cases, is often linked to low sperm count, poor motility, and abnormal morphology. The study explores whether probiotics can reduce oxidative stress and DNA fragmentation that negatively affect sperm function and fertilization potential. The study is a randomized, placebo-controlled pilot trial involving 60 male participants aged 20 to 45 years. Participants are randomly assigned to receive either probiotics or a placebo, taking two capsules daily after meals for approximately 100 days. Semen samples are collected at the start, mid-point (Day 60), and end (Day 100) of the intervention to assess sperm concentration, motility, morphology, DNA integrity, and function. During the study, sperm concentration, motility, and morphology are measured using Computer-Aided Sperm Analysis (CASA). Secondary assessments include sperm DNA fragmentation measured by the Sperm Chromatin Structure Assay (SCSA) and sperm function evaluated by the acrosome reaction assay. These tests help determine changes over time. The study includes careful monitoring and aims to clarify probiotics' potential role in improving male infertility. Participation lasts about 100 days with sample collections and evaluations at three specific time points.

CONDITIONS

Brief Title

Probiotics on Sperm Quality in Male Infertility Patients

Who Can Participate

Age: 20Years - 45Years
MALE

Eligibility Criteria

Eligible

You may qualify if you...

  • Males aged 20 to 45 years
  • Diagnosed with unexplained oligozoospermia, asthenozoospermia, or oligoasthenoteratozoospermia
  • Sperm concentration less than 5 x 10^6/mL
  • Sperm total motility less than 40%
  • Sperm morphology (Kruger strict criteria) less than 4%
Not Eligible

You will not qualify if you...

  • History of hormonal disorders or epididymo-orchitis
  • Substance abuse, including drugs or excessive alcohol consumption
  • Diabetes mellitus
  • Kidney disease defined as a doubling of creatinine levels or more
  • Chronic liver disease
  • Varicocele
  • Current use of medications that interfere with hormones
  • Occupational or environmental exposure to pesticides, heavy metals, or solvents
  • Intake of antioxidant supplements within the past three months
  • Body Mass Index (BMI) of 30 kg/m^2 or higher

AI-Screening

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Your Study Journey

Screening

Duration - 2 to 4 weeks

Participants are screened for eligibility to participate in the trial.

1 visit (in-person)

Treatment

Duration - Approximately 100 days

Participants take either probiotic capsules or placebo capsules daily to evaluate the effect on sperm quality.

3 visits (Day 0, Day 60, Day 100) for semen sample collection

Trial Site Locations

Total: 1 location

1

MacKay Memorial Hospital

New Taipei City, Taiwan, 25160

Actively Recruiting

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Research Team

S

Sheng-Hsiang Li, PhD

How is the study designed?

Study Type

INTERVENTIONAL

Masking

QUADRUPLE

Allocation

RANDOMIZED

Model

PARALLEL

Primary Purpose

TREATMENT

Number of Arms

2

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