Study of Probiotics to Improve Sperm Quality in Men with Infertility Aged 20 to 45

What this Trial Is About

Researchers are evaluating the impact of probiotic supplements on sperm quality in men diagnosed with infertility, focusing on unexplained oligozoospermia, asthenozoospermia, or oligoasthenoteratozoospermia. Male infertility, which accounts for about 40% of infertility cases, is often linked to low sperm count, poor motility, and abnormal morphology. The study explores whether probiotics can reduce oxidative stress and DNA fragmentation that negatively affect sperm function and fertilization potential. The study is a randomized, placebo-controlled pilot trial involving 60 male participants aged 20 to 45 years. Participants are randomly assigned to receive either probiotics or a placebo, taking two capsules daily after meals for approximately 100 days. Semen samples are collected at the start, mid-point Day 60, and end Day 100 of the intervention to assess sperm concentration, motility, morphology, DNA integrity, and function. During the study, sperm concentration, motility, and morphology are measured using Computer-Aided Sperm Analysis CASA. Secondary assessments include sperm DNA fragmentation measured by the Sperm Chromatin Structure Assay SCSA and sperm function evaluated by the acrosome reaction assay. These tests help determine changes over time. The study includes careful monitoring and aims to clarify probiotics potential role in improving male infertility. Participation lasts about 100 days with sample collections and evaluations at three specific time points.

Probiotics on Sperm Quality in Male Infertility Patients