Studying Biomarkers to Predict Success of Full Pulpotomy and Root Canal Treatments in Permanent Teeth with Irreversible Pulpitis

What this Trial Is About

Researchers are investigating treatments for permanent mature molars diagnosed with irreversible pulpitis, a painful dental condition. The study aims to identify biomarkers that can predict the outcome of pulpotomy, a dental procedure removing diseased pulp tissue. It compares the long-term success of full pulpotomy with root canal treatment RCT over a two-year period, using blood and tissue samples to study inflammation and cellular activity. Participants will receive either full pulpotomy, where the entire roof of the pulp chamber and pulp tissue are removed and covered with Biodentine before restoring the tooth with resin-based composite, or root canal treatment as a control. Blood samples and pulp tissues will be collected during treatment for detailed analysis including histology, spatial imaging, and single-cell RNA sequencing. Pulpotomy outcomes will be assessed clinically and with radiographs at 6 and 12 months, while the comparison with RCT outcomes will occur at 24 months. During the study, researchers will monitor treatment success and failure by analyzing biomarkers in blood and tissue samples and correlating these with clinical outcomes. Participants will undergo evaluations at specific intervals to track healing and treatment effectiveness. The study includes 180 molars 120 treated with pulpotomy and 60 with RCT, plus samples from healthy teeth as controls. This research spans two years, with outcomes measured at multiple time points to better understand and improve treatment for irreversible pulpitis.

Prognostic Biomarkers for Pulpotomy Outcome