Study of Tumor Changes During Breast Cancer Surgery Collecting Tumor Tissue Samples Before, During, and After Surgery

What this Trial Is About

This research aims to understand the effects of acute hypoxia that occurs during breast cancer surgery. Researchers will study changes in the tumor caused by oxygen deprivation during the operation by collecting tumor tissue samples at different times during surgery. The study focuses specifically on how gene expression in the tumor changes during the surgical process, which lasts about 30 to 90 minutes on average. During surgery, three tumor samples will be taken while the patient is under anesthesia one before surgery starts, one midway when half the tumor has been devascularized, and one after the tumor is fully removed. These samples will be preserved using methods like snap freezing, RNA later storage, and paraffin sections for future genomic and protein studies. Participants will have tumor tissue biopsies collected only during surgery without any additional clinical follow-up after sampling. Researchers will evaluate gene expression changes and use immunohistochemistry to study other markers in the tumor tissue. The entire process occurs during the surgical procedure without extended monitoring or post-surgery data collection.

A Prospective Evaluation of the Peri-operative Hypoxia in Breast Cancer