Group A

• Patients with normal menstrual cycles lasting 28-34 days
• Patients with normal uterine anatomy
• Patients with no prior pregnancy history including miscarriages, terminations, ectopic pregnancies, preterm deliveries, or full-term deliveries
• Patient who have no known infertility (i.e. women who have not tried to conceive)

• BMI ≥ 35
• Current use of intrauterine devices (IUDs), hormonal implants, or hormonal birth control medications. Participants on hormonal birth control interested in study participation may be included following one month of discontinuation of hormonal medication.
• History of uterine surgery (i.e hysteroscopy, dilation and curettage, myomectomy)
• History of ovarian surgery (i.e ovarian cystectomy, oophorectomy, removal of -endometrioma)
• History of surgically-confirmed or clinically suspected endometriosis or ultrasound evidence of endometriosis (i.e endometrioma)
• Currently present or surgically corrected uterine anomalies
• Ultrasound evidence of or history of communicating hydrosalpinx
• Ultrasound evidence of or history of leiomyomas
• Ultrasound evidence of adenomyosis based on the Morphological Uterus Sonographic Assessment criteria (MUSA) (19)
• Ultrasound evidence of ovarian pathology including mature teratoma/dermoid cyst, persistent functional cysts larger than 2 cm, or ovarian cancer.

Group B

Inclusion Criteria:

• Patients with normal menstrual cycles lasting 28 days to 34 days.
• Patients with normal uterine and ovarian anatomy (absence of gross pathology on screening visit ultrasound)
• Patients with at least one prior full-term vaginal delivery with time to conception less than one year without the use of assisted reproductive technology.

Exclusion Criteria

• BMI ≥ 35
• Current use of intrauterine devices (IUDs), hormonal implants, or hormonal birth control medications. Participants on hormonal birth control interested in study participation may be included following one month of discontinuation of hormonal medication.
• History of cesarean section
• Patient who have no known secondary infertility (i.e. women who have not tried to conceive)
• Diagnosis of secondary infertility (i.e women who have attempted pregnancy for 12 months without success)
• History of uterine surgery (i.e hysteroscopy, dilation and curettage, myomectomy)
• History of ovarian surgery (i.e ovarian cystectomy, oophorectomy, removal of endometrioma)
• History of surgically-confirmed or clinically suspected endometriosis or ultrasound evidence of endometriosis (i.e endometrioma)
• Currently present or surgically corrected uterine anomalies
• Ultrasound evidence of or history of communicating hydrosalpinx
• Ultrasound evidence of or history of leiomyomas
• Ultrasound evidence of adenomyosis based on the Morphological Uterus Sonographic Assessment criteria (MUSA) (19)
• Ultrasound evidence of ovarian pathology including mature teratoma/dermoid cyst, persistent functional cysts larger than 2 cm, or ovarian cancer.

Group C

Inclusion Criteria:

• Patients with normal menstrual cycles lasting 28 days to 34 days.
• Patients with normal uterine and ovarian anatomy (absence of gross pathology on screening visit ultrasound)
• Patient with no prior pregnancy history including miscarriages, terminations, ectopic pregnancies, preterm deliveries, or full-term deliveries (i.e gravity equals zero).
• Patient with diagnosis of primary infertility, with attempted conception for at least 12 months if younger than 35 years and attempted conception for at least 6 months if 35 years or older.

Exclusion Criteria

• BMI ≥ 35
• Current use of intrauterine devices (IUDs), hormonal implants, or hormonal birth control medications. Participants on hormonal birth control interested in study participation may be included following one month of discontinuation of hormonal medication.
• History of three or more failed euploid embryo transfers
• Severe male factor infertility including severe oligozoospermia and cryptozoospermia
• History of uterine surgery (i.e hysteroscopy, dilation and curettage, myomectomy)
• History of ovarian surgery (i.e ovarian cystectomy, oophorectomy, removal of endometrioma)
• History of surgically-confirmed or clinically suspected endometriosis or ultrasound evidence of endometriosis (i.e endometrioma)
• Currently present or surgically corrected uterine anomalies
• Ultrasound evidence of or history of communicating hydrosalpinx
• Ultrasound evidence of or history of leiomyomas
• Ultrasound evidence of adenomyosis based on the Morphological Uterus Sonographic Assessment criteria (MUSA) (19)
• Ultrasound evidence of ovarian pathology including mature teratoma/dermoid cyst, persistent functional cysts larger than 2 cm, or ovarian cancer.