Actively Recruiting

Phase 2
Phase 3
Age: 3Years - 8Years
All Genders
Healthy Volunteers
NCT07450443

Role of the Gut Vascular Barrier and Microbiota in Autism Spectrum Disorders

Led by Fondazione I.R.C.C.S. Istituto Neurologico Carlo Besta · Updated on 2026-03-04

90

Participants Needed

2

Research Sites

245 weeks

Total Duration

On this page

Sponsors

F

Fondazione I.R.C.C.S. Istituto Neurologico Carlo Besta

Lead Sponsor

I

Istituto Clinico Humanitas

Collaborating Sponsor

AI-Summary

What this Trial Is About

Recent research links gut microbiota alterations to Autism Spectrum Disorders (ASD), a neurobiological condition with multifactorial bases. In some ASD patients, altered gut flora and increased intestinal permeability are observed, influencing the central nervous system's development and function. Chronic gastrointestinal (GI) symptoms are commonly associated with ASD and correlate with its severity. This non-pharmacological interventional clinical study aims to investigate the role of gut microbiota on ASD and the effectiveness of postbiotic-based dietary supplements in children aged 3-8 years old. Gastrointestinal symptoms, behavioral profile and analysis of intestinal metagenomic and metabolomic profiles will be assessed before and after one-month treatment. The results of the study could enhance understanding of non-pharmacological therapeutic approaches in ASD and improve clinical management strategies and the behavioural functioning for children with ASD.

CONDITIONS

Official Title

Role of the Gut Vascular Barrier and Microbiota in Autism Spectrum Disorders

Who Can Participate

Age: 3Years - 8Years
All Genders
Healthy Volunteers

Eligibility Criteria

Eligible

You may qualify if you...

  • Children aged 3 to 8 years with a diagnosis of ASD based on DSM-5 criteria
  • Clinical neurological and neuropsychological evaluation supporting ASD diagnosis with standardized instruments
  • Assessment of psychomotor or intellectual development using recognized scales
  • Presence of at least one gastrointestinal symptom (such as constipation, diarrhea, abnormal stool consistency, flatulence, abdominal pain, irritability, or abdominal tenderness) with a minimum severity score
  • Signed informed consent for microbiota analysis and postbiotic supplement treatment
  • Typically developing children aged 3 to 8 years without gastrointestinal symptoms for control group
  • Signed informed consent for microbiota analysis for control group
Not Eligible

You will not qualify if you...

  • Children with syndromic ASD or known genetic diseases
  • Children with serious health problems requiring surgery or continuous medical treatment
  • Severe gastrointestinal problems needing immediate life-threatening treatment
  • Severely underweight or malnourished children
  • Use of antibiotics, probiotics, or prebiotics within 1 month before enrollment
  • Participants with gastrointestinal symptoms requiring immediate treatment or chronic GI issues such as constipation, diarrhea, encopresis, bloating, reflux, vomiting, or food aversion in control group

AI-Screening

AI-Powered Screening

Complete this quick 3-step screening to check your eligibility

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Trial Site Locations

Total: 2 locations

1

Fondazione IRCCS Istituto Neurologico Carlo Besta

Milan, MI, Italy, 20133

Actively Recruiting

2

IRCCS Humanitas Reasearch Hospital (ICH) Laboratory of Microbiota and Mucosal Immunology

Milan, Italy

Actively Recruiting

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Research Team

S

Stefano D'Arrigo, MD

CONTACT

S

Sara Bulgheroni

CONTACT

How is the study designed?

Study Type

INTERVENTIONAL

Masking

NONE

Allocation

NON_RANDOMIZED

Model

PARALLEL

Primary Purpose

TREATMENT

Number of Arms

3

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