Actively Recruiting

Age: 18Years +
All Genders
Healthy Volunteers
NCT07141758

Salivary E-cadherin, Calprotectin, and Matrix Metalloproteinase-9 Levels in Recurrent Aphthous Stomatitis

Led by Selin Yeşiltepe · Updated on 2025-08-26

102

Participants Needed

1

Research Sites

63 weeks

Total Duration

On this page

Sponsors

S

Selin Yeşiltepe

Lead Sponsor

A

Aydın Adnan Menderes University Scientific Research Projects Unit

Collaborating Sponsor

AI-Summary

What this Trial Is About

The goal of this observational cross-sectional study is to determine the salivary levels of E-cadherin, Calprotectin, and Matrix Metalloproteinase-9 (MMP-9) in patients with Recurrent Aphthous Stomatitis (RAS) and to compare them with those of healthy controls. The main questions it aims to answer are: Are the salivary levels of E-cadherin, Calprotectin, and MMP-9 significantly different in patients with minor RAS compared to healthy controls? Can these salivary proteins serve as biomarkers involved in the pathogenesis of minor RAS? Researchers will compare patients with minor RAS and healthy individuals to determine whether differences in salivary E-cadherin, Calprotectin, and MMP-9 levels are associated with the presence of disease. Participants will: Provide unstimulated saliva samples using a saliva swab Undergo clinical evaluation for periodontal status (Community Periodontal Index - CPI) and caries experience (DMFT index) Collected saliva samples will be stored at -80°C until analysis. The concentrations of the investigated proteins will be measured using ELISA kits, and total protein levels will be determined using a colorimetric protein assay. The relationships among the salivary concentrations of the investigated proteins, as well as their associations with demographic variables such as age and sex, will be analyzed.

CONDITIONS

Official Title

Salivary E-cadherin, Calprotectin, and Matrix Metalloproteinase-9 Levels in Recurrent Aphthous Stomatitis

Who Can Participate

Age: 18Years +
All Genders
Healthy Volunteers

Eligibility Criteria

Eligible

You may qualify if you...

  • Participants aged 18 years or older
  • Clinically diagnosed with minor recurrent aphthous stomatitis (at least three episodes in the past year)
  • Systemically healthy without any diagnosed systemic disease
  • No history of tobacco use
  • Presence of at least one active aphthous ulcer at the time of saliva collection
  • No known mental or physical disabilities
Not Eligible

You will not qualify if you...

  • Use of any pharmacological treatment within the past 3 months
  • Use of antiseptic mouthwash within the past 3 months
  • Pregnant, lactating, or currently using oral contraceptives
  • History of systemic diseases linked to oral ulcers such as Crohn's disease, ulcerative colitis, celiac disease, or Behçet's disease
  • History of chemotherapy or radiotherapy
  • Current or past use of immunomodulatory drugs, cytotoxic agents, or systemic corticosteroids
  • Undergoing orthodontic treatment
  • Use of removable dental prostheses
  • Presence of periodontal disease with CPI score of 3 or 4
  • Caries risk not very low (DMFT 5 or higher)
  • Presence of pathological oral mucosal lesions such as lichen planus, pemphigus vulgaris, or mucous membrane pemphigoid

AI-Screening

AI-Powered Screening

Complete this quick 3-step screening to check your eligibility

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Trial Site Locations

Total: 1 location

1

Aydın Adnan Menderes University

Aydin, Efeler, Turkey (Türkiye), 09010

Actively Recruiting

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Research Team

S

Sunay Hoşgör, dds

CONTACT

S

Selin Yeşiltepe, dds,msd

CONTACT

How is the study designed?

Study Type

OBSERVATIONAL

Masking

N/A

Allocation

N/A

Model

N/A

Primary Purpose

N/A

Number of Arms

2

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