Actively Recruiting

Age: 18Years - 120Years
All Genders
ID04807114

A Single-cell Approach to Identify Biomarkers of Efficacy and Toxicity for Immune Checkpoint Blockade in Non-small Cell Lung Cancer

Led by Universitaire Ziekenhuizen KU Leuven · Updated on 2024-07-01

70

Participants Needed

1

Research Sites

N/A

Total Duration

On this page

Sponsors

U

Universitaire Ziekenhuizen KU Leuven

Lead Sponsor

K

KU Leuven

Collaborating Sponsor

AI-Summary

What this Trial Is About

Researchers are studying advanced non-small cell lung cancer (NSCLC) patients to better understand how the immune system interacts with tumors before treatment with immune checkpoint inhibitors (ICI). This observational study aims to identify new biomarkers related to how well patients respond to these therapies and explore mechanisms behind resistance. They also seek to find markers for immune-related side effects like ICI-pneumonitis by analyzing immune cells in blood and lung fluid samples. The study collects tumor biopsies from 70 stage IV NSCLC patients before they start treatment with ICIs. These samples undergo detailed single-cell RNA and TCR sequencing to identify different immune and stromal cell types and their gene activity. Blood samples are similarly analyzed to compare immune cell profiles. For patients developing ICI-related lung inflammation, bronchoalveolar lavage fluid will also be studied to find diagnostic markers. Treatments include either anti-PD-1 monotherapy or combination of anti-PD-1 with chemotherapy, according to standard care based on PD-L1 levels. Participants will be monitored from inclusion through about two years, during which immune cell proportions in tumor biopsies, blood, and lung fluid will be measured and correlated with treatment response. Researchers will use advanced bioinformatics to identify immune cell clusters and gene programs to understand immune activation and suppression. This comprehensive monitoring aims to develop new non-invasive biomarkers to guide future treatment and management of NSCLC patients receiving immune checkpoint therapies.

CONDITIONS

Brief Title

A Single-cell Approach to Identify Biomarkers of Efficacy and Toxicity for ICI in NSCLC

Who Can Participate

Age: 18Years - 120Years
All Genders

Eligibility Criteria

Eligible

You may qualify if you...

  • Adults aged 18 years or older
  • Histologically and clinically confirmed diagnosis of non-small cell lung cancer
  • Patients receiving first-line treatment according to guidelines
  • Not currently enrolled in any other clinical trials
  • Signed informed consent form
Not Eligible

You will not qualify if you...

  • Collected material not suitable for further processing due to poor quality, as determined by specialized lab staff

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Your Study Journey

Screening

Duration - 2 to 4 weeks

Participants are screened for eligibility to participate in the trial.

1 screening and enrollment visit

Diagnostic Evaluation

Duration - Within 1 week before treatment start

Participants undergo routine medically required tumor biopsies and blood sampling to collect samples for experimental single-cell analyses.

1 visit (in-person) for tumor biopsy and blood sampling

Monitoring

Duration - Up to approximately 2 years

Participants who receive standard-of-care immune checkpoint inhibitor treatment (with or without chemotherapy) are observed to assess immune cell profiles in tumor and blood samples over time.

Periodic sample collections and assessments as per routine care

Trial Site Locations

Total: 1 location

1

Universitaire Ziekenhuizen Leuven

Leuven, Belgium, 3000

Actively Recruiting

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Research Team

E

Els Wauters

How is the study designed?

Study Type

OBSERVATIONAL

Masking

N/A

Allocation

N/A

Model

N/A

Primary Purpose

N/A

Number of Arms

2

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