Actively Recruiting

Phase 2
Phase 3
Age: 18Years +
All Genders
Healthy Volunteers
NCT05653258

Single Nuclei RNA-seq to Map Adipose Cellular Populations and Senescent Cells in Older Subjects

Led by Cedars-Sinai Medical Center · Updated on 2026-03-18

160

Participants Needed

1

Research Sites

224 weeks

Total Duration

On this page

Sponsors

C

Cedars-Sinai Medical Center

Lead Sponsor

N

National Institute on Aging (NIA)

Collaborating Sponsor

AI-Summary

What this Trial Is About

All participants will undergo baseline biopsies of subcutaneous abdominal adipose tissue for cellular/molecular profiling via snRNA-seq and metabolic/physiological assessments (insulin sensitivity, glucose tolerance, and β-cell function). Older obese participants will be randomized into three arms: lifestyle intervention (n=24), senolytics (n=24), or placebo (n=24).

CONDITIONS

Official Title

Single Nuclei RNA-seq to Map Adipose Cellular Populations and Senescent Cells in Older Subjects

Who Can Participate

Age: 18Years +
All Genders
Healthy Volunteers

Eligibility Criteria

Eligible

You may qualify if you...

  • Both sexes are eligible
  • Age 18-30 years with BMI 18.5-24.9 kg/m2 (younger lean group)
  • Age 18-30 years with BMI 30.0-39.9 kg/m2 (younger obese group)
  • Age 65 years or older with BMI 30.0-39.9 kg/m2 (older obese group)
  • All races and ethnic groups
  • Living in the community
  • Sedentary lifestyle (1.5 hours or less of exercise per week)
  • Nondiabetic with fasting plasma glucose less than 126 mg/dl, 2-hour glucose during oral glucose tolerance test less than 140 mg/dl, and HbA1c less than 6.5%
  • Women of childbearing potential must be not pregnant or breastfeeding and use highly effective contraception during the study and for 30 days after
Not Eligible

You will not qualify if you...

  • Diagnosed diabetes or HbA1c greater than 6.5%, fasting plasma glucose greater than 126 mg/dl, or use of anti-diabetic medications
  • Exercising more than 1.5 hours per week
  • Unstable weight with more than 3% change in the last 3 months
  • Neurological, musculoskeletal, or other conditions limiting physical assessment or training
  • Active autoimmune or inflammatory diseases such as rheumatoid arthritis, multiple sclerosis, lupus, or inflammatory bowel disease
  • Abnormal lab results indicating impaired kidney or liver function, abnormal thyroid stimulating hormone, low hemoglobin or platelets, or abnormal blood clotting tests
  • Active gastrointestinal disease, bleeding disorders, or recent gastrointestinal bleeding within 6 months
  • Significant heart disease or peripheral vascular disease
  • QTc prolongation over 45 milliseconds or use of medications that prolong QTc
  • Pulmonary disease including chronic obstructive pulmonary disease, severe asthma, or exercise-induced asthma
  • Recent blood clots or embolism
  • Uncontrolled high blood pressure (systolic over 170 mmHg or diastolic over 95 mmHg)
  • Smoking, excessive alcohol use, or recreational drug use (other than marijuana)
  • Pregnant or breastfeeding
  • Recent start of systemic hormone replacement therapy in postmenopausal women
  • Previous bariatric surgery
  • History of stroke with motor disability
  • Recent treated cancer within 3 years (excluding basal cell carcinoma)
  • Acute or chronic infections
  • Use of medications that interfere with metabolic studies or senolytic agents within 6 months
  • History of allergy to dasatinib, quercetin, or lidocaine
  • Concurrent participation in another interventional trial

AI-Screening

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Trial Site Locations

Total: 1 location

1

Cedars Sinai Medical Center

Los Angeles, California, United States, 90048

Actively Recruiting

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Research Team

N

Nicolas Musi, MD

CONTACT

A

Arianne Aslamy, MD

CONTACT

How is the study designed?

Study Type

INTERVENTIONAL

Masking

DOUBLE

Allocation

RANDOMIZED

Model

PARALLEL

Primary Purpose

TREATMENT

Number of Arms

4

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