Investigating the Link Between Endometriosis and Tissue Microbiota in Women Aged 18 to 52

What this Trial Is About

Endometriosis is a common gynecological disease affecting women of reproductive age, causing pain, masses, and infertility. The exact cause of endometriosis is unclear. Researchers are examining the relationship between endometriosis and the microbiota found within tissue to better understand this condition. This observational study compares women diagnosed with endometriosis to those without it, often having other benign gynecologic conditions like uterine fibroids or teratomas. Participants will have samples collected before surgery, including blood serum, vaginal swabs, cervical mucus, and feces. During surgery, if possible, additional samples such as peritoneal fluid and both eutopic and ectopic endometrial tissue will be taken. These samples will undergo various sequencing methods like microbiota, transcriptome, metabolome, and single-cell sequencing to analyze microbial composition and related mechanisms. During the study, participants will be evaluated through surgical confirmation and biopsy analysis. Researchers will measure the composition of intra-tissue microbiota using metagenomics next-generation sequencing over a three-year period. The study includes detailed sample collection and laboratory experiments to explore the microbiotas role in endometriosis, with participation lasting from screening through surgical and laboratory procedures.

A Study on the Correlation Between Endometriosis and Intra-tissue Microbiota