Study of Bone Marrow Environment in Childhood Acute Leukemia Including Acute Lymphoid and Myeloid Leukemia In Children Aged 1 to 15 Years

What this Trial Is About

Acute leukemia is the most common cancer in children and can sometimes relapse or lead to death despite improvements in chemotherapy and supportive care. This research focuses on better understanding the diseases biology, especially how the bone marrow environment affects leukemia cells and their resistance to treatment. The study highlights the importance of the bone marrow microenvironment, including mesenchymal stromal stem cells, and their role in leukemia growth and chemotherapy resistance in children. The study involves two groups children diagnosed with acute lymphoid leukemia B or T, or acute myeloid leukemia, and a control group of children without blood diseases undergoing orthopedic surgery exposing bone marrow. Researchers will collect blood and bone marrow samples from both groups to study the energy metabolism and other cellular characteristics of mesenchymal stromal stem cells. The study is non-randomized and does not involve masking or blinding. Participants will have biological samples taken at inclusion, which will be analyzed for oxygen consumption rate and other measures such as acidification rate, reactive oxygen species, cell doubling time, immunophenotypic profiles, mutational profiles, transcriptomic signatures, and cytokine profiles within the bone marrow. The study will monitor these outcomes to better understand how the bone marrow environment influences acute childhood leukemia. Participation requires parental consent and social security affiliation, with a total age range of 1 to 15 years old.

Study of the Medullary Microenvironment in Acute Childhood Leukemia