Using Joint Fluid and Tissue Samples to Improve Diagnosis of Bone and Joint Diseases An Observational Study on Biomarkers in Rheumatic Conditions

What this Trial Is About

This research aims to improve early diagnosis and classification of rheumatic diseases, which affect bones and joints. Rheumatic diseases are typically diagnosed using clinical signs, biological tests, and imaging, but in about 20% of cases, the diagnosis remains unclear, delaying treatment. Conventional methods may lack sensitivity and specificity for early detection, so the study explores novel biomarkers from biological samples to assist diagnosis and patient classification. The study collects biological samples such as synovial fluid via joint aspiration, blood through venous puncture, and tissue samples during surgery, which are part of routine patient care. These samples will be analyzed using both conventional laboratory tests like histology, immunodetection, and PCR, as well as innovative techniques such as Raman spectroscopy and nanospectroscopy. The investigators aim to detect new biomarkers that can better characterize rheumatic diseases and their progression. Participants provide samples during their routine care procedures, and researchers analyze the chemical, biological, and protein changes in these samples using advanced laboratory methods. The primary outcomes include spectral analysis of tissues and biofluids with surface-enhanced Raman spectroscopy, and protein and RNA expression using immunodetection and PCR techniques. This observational study involves ongoing data collection until 2029, with no direct intervention beyond sample collection during standard medical procedures.

"Translating Articular Biomarkers Into Diagnoses"