Pilot Study Evaluating Effects of L-Phenylalanine Supplement on Gut Fungal Populations and Phenylpropionic Acid in Healthy Adults

What this Trial Is About

Researchers are investigating how the dietary supplement L-Phenylalanine affects the production of phenylpropionic acid PPA and changes in fungal populations in the gut microbiome of healthy adults. The focus is on understanding the interactions between gut fungi and metabolites produced by gut bacteria, particularly how PPA influences fungal growth. This pilot study aims to explore these relationships in a basic science context using healthy volunteers. Participants will receive a 14-day supply of L-Phenylalanine capsules, taking two 500 mg capsules in the morning and one 500 mg capsule in the evening daily. Stool and blood samples will be collected before supplementation and at Day 14 to assess changes. The study evaluates the impact of L-Phenylalanine on gut fungal populations, especially Candida species, and on PPA levels produced by gut bacteria. During the study, participants provide stool and blood samples at baseline and after the 14-day supplement period. Researchers will measure changes in phenylpropionic acid levels in fecal material, shifts in fungal populations including Candida, and changes in T cells reacting to fungal antigens. Additional measurements occur at Day 28 to monitor ongoing effects. The overall participation involves sample collection and supplement intake over two weeks, with follow-up assessments to understand the supplements influence on gut microbiome dynamics.

Understanding the Efficacy of Dietary Supplement on Fungal Mycobiota in Healthy Volunteers: A Pilot Study