Evaluating SDS-EDTA-Treated Paper Strips for Collecting and Testing Mpox Virus Samples in the Democratic Republic of the Congo

What this Trial Is About

Researchers are evaluating a new method using SDS-EDTA-treated chromatography paper strips for collecting and transporting samples to detect mpox virus. This observational study compares this method to the standard swab-based sampling among patients with suspected mpox infection in healthcare facilities in the Democratic Republic of the Congo. The aim is to assess the diagnostic accuracy and practical use of the strip method in field conditions. Participants will have paired samples taken simultaneously using both the standard swab method and the SDS-EDTA strip method from skin lesions suitable for sampling. The chromatography strip contains SDS, which inactivates the virus to reduce biosafety risks during transport, and EDTA, which stabilizes nucleic acids and lessens the need for cold storage. Samples collected are analyzed using local molecular diagnostic platforms. During the study, participants will be assessed based on their clinical signs and suitability of lesions for paired sampling. Researchers will measure the difference in mpox virus detection between the two sampling methods through laboratory analysis of samples collected up to six months after collection. Safety and consent procedures are in place, and total participation duration depends on sample collection and analysis timelines.

Validation of SDS-EDTA-Treated Chromatography Paper Strips for Mpox Sampling and Transport in DRC