Use of SDS/EDTA-Treated Chromatography Strips for Sampling, Transportation and Laboratory Confirmation of Mpox Virus Infection

What this Trial Is About

Researchers are evaluating a new method for collecting and transporting samples to detect mpox virus infection. This study compares SDS-EDTA-treated chromatography paper strips with the routine swab-based sampling method to see which is better at identifying the virus under real field conditions in the Democratic Republic of the Congo. The study focuses on patients suspected of having mpox who visit healthcare facilities in South Kivu, DRC. Participants will have paired samples taken at the same time using both the standard swab method and the SDS-EDTA strip method. The SDS-EDTA strip contains chemicals that inactivate the virus and stabilize its genetic material, making transport safer and less dependent on refrigeration. Both types of samples are then analyzed using molecular diagnostic tools available locally. During the study, researchers collect samples from patients with at least two skin lesions suitable for paired sampling. The main goal is to compare how often each sampling method detects the mpox virus. Laboratory analysis of the samples may continue for up to six months after they are collected. Participants provide written consent, and the study monitors the diagnostic accuracy and practicality of the new strip method in field settings.

Validation of SDS-EDTA-treated Chromatography Paper Strips for Sampling, Transportation and Laboratory Detection of Mpox Under Field Conditions in the Democratic Republic of the Congo