Study of Vitamin B12, Folic Acid, and Vitamin D Levels in Women Aged 18 to 49 to Improve Supplementation Guidelines Before and During Pregnancy

What this Trial Is About

Researchers are evaluating the serum levels of vitamin B12, folic acid, and vitamin D in women of reproductive age living in Herzegovina. The study aims to find out whether current international guidelines for micronutrient supplementation during the preconception period and pregnancy accurately reflect the needs of this population. This observational, cross-sectional study is led by Professor Vajdana Tomi at the Faculty of Health Studies, University of Mostar, Bosnia and Herzegovina. The study will include 360 women aged 18 to 49 years, who will provide blood samples for analysis of vitamin B12, folic acid, and vitamin D levels. No treatments or interventions will be given. The study is conducted in three phases recruitment and sample collection, laboratory analysis using chemiluminescent microparticle immunoassays on the Alinity ci platform, and data evaluation followed by publication of results. The goal is to develop population-specific recommendations for micronutrient supplementation. Participants will attend a single visit for blood sampling to measure serum concentrations of the studied vitamins. Researchers will analyze the samples and use statistical software to assess the prevalence of micronutrient deficiency or excess. The study will monitor safety and adherence through informed consent and standard procedures. Results will guide personalized supplementation strategies for women planning pregnancy or already pregnant, with the total participation lasting one visit.

Vitamin B12, Folic Acid and Vitamin D Status in Women of Reproductive Age