Lateral anal fissure

Presence of peri-anal or rectovaginal fistula, rectal or anal stenosis, or peri-anal abscess or non-healing peri-anal post-surgical wounds that are not anal fissures (subjects with history of anorectal surgery with healed surgical wound is not excluded)

Active, untreated or medically unresponsive infection of the anal fissure or fistula (e.g., erythema and pus)

Active systemic infection (e.g., bacteremia, sepsis) - stable, controlled and treated HIV+ subjects (e.g., recent plasma HIV RNA <200 copies/mL) are not excluded

Presence of inflammatory bowel diseases (e.g., Crohn's, ulcerative colitis)

Taking systemic chemotherapy or local pelvic radiation treatments

Renal impairment defined by serum creatinine ≥ 1.5 x upper limit of normality (ULN)

Hepatic impairment defined by both of the following laboratory ranges:

(a) total bilirubin ≥ 1.5 x ULN unless benign congenital hyperbilirubinemia; and (b) aspartate aminotransferase (AST) and alanine aminotransferase (ALT) ≥ 2.5 x ULN

Active alcohol or substance use that, in the opinion of the site investigator, will interfere with study follow-up.

Active malignant tumor (tumors must be in remission for ≥ 6 months without maintenance chemotherapy and/or radiation)

Ongoing or recent history (within 6 months) of abnormal, severe, progressive, or uncontrolled hepatic, hematological, gastrointestinal, endocrine, pulmonary, cardiac, neurological, psychiatric, or cerebral diseases

Congenital immunodeficiencies

History of major surgery or severe trauma within the previous 3 months

Subjects who are actively being considered as candidates for solid organ transplantation or who may have a high likelihood of needing a solid organ transplant (ex. Progressive heart failure)

Females who are pregnant or breastfeeding or planning to become pregnant or breastfeed during the study (180 days)

Subjects who have known hypersensitivity or documented allergy to DMSO

Subjects who do not wish to or cannot comply with study procedures