Molecular Biology (Multiplex PCR) in the Identification of Pathogens at Reimplantation in the Two-Stage Treatment of Periprosthetic Infections (PJI)

What this Trial Is About

Researchers are evaluating the accuracy of the Multiplex PCR technique to identify pathogens during the diagnostic stage of resolving pre-implantation infection in patients undergoing two-stage treatment for periprosthetic joint infections (PJIs). This observational study compares results from Multiplex PCR with those from standard culture examinations to understand how well the PCR method detects infection-causing bacteria. The study gathers detailed information about patients with PJIs, including their medical history, infection timing, and surgical details. Participants will be observed through several stages. Data collected include pre-implantation details such as infection timing, bacterial types, surgery type, patient characteristics, and lifestyle habits. During explant surgery and the postoperative period, information about spacer cement use, complications, antibiotic therapy, and molecular and culture test results will be recorded. At reimplantation, further molecular, culture, and histological tests will be done, along with monitoring antibiotic use, complications, and clinical status over follow-up. Throughout the study, participants will undergo various assessments including laboratory and molecular tests, histological exams, and clinical evaluations to track infection status and recovery. Researchers will measure the accuracy, sensitivity, and specificity of the PCR technique up to 100 weeks. The study follows participants from pre-implantation through reimplantation and subsequent follow-up to monitor for infection recurrence or persistence, documenting all relevant clinical and laboratory findings.

Molecular Biology in the Identification of Pathogens at Reimplantation in the Two-Stage Treatment of Periprosthetic Infections