Study Comparing Genetic Profiles of Primary Colorectal Cancer Tumors and Their Brain Metastases

What this Trial Is About

Researchers are investigating the molecular genetic similarity between primary tumors and brain metastases in patients with colorectal cancer. This observational study focuses on patients who have developed brain metastases, a rare and serious complication occurring in less than 3% of colorectal cancer cases. The study aims to better understand the genetic profiles of these tumors by analyzing specific gene mutations and their concordance between original and metastatic sites. The study involves molecular genetic testing of archival tumor samples collected from neurosurgical resections. Researchers will analyze mutations in KRAS, NRAS, BRAF, HER2 genes, and microsatellite instability MSI using specialized sequencing and immunohistochemical methods. This testing compares paired samples from both the primary colorectal tumor and its brain metastases to calculate how closely their genetic profiles match. Participants tumor samples will be examined for mutations and gene expressions using advanced laboratory techniques. The main outcome measured is the concordance rate of genetic mutations between the two tumor sites within one month. Secondary outcomes include monitoring intracranial progression-free survival and overall survival at intervals up to six months. The study involves no active treatment but detailed genetic and clinical follow-up over time to improve understanding of metastatic colorectal cancer biology.

Comparison of Molecular-Genetic Concordance of the Primary Tumor and Brain Metastases of Colorectal Cancer