Study of Sodium Fluoride and Chlorhexidine Effects on Saliva Biomarkers in Children Aged 6 to 8 With and Without Active Tooth Decay

What this Trial Is About

This research aims to compare levels of specific salivary biomarkersIL-6, MMP-8, MMP-9, and TIMP-1between healthy children and children with active dental caries. The study focuses on understanding the role of inflammation and tissue breakdown in caries progression and evaluates how 5% sodium fluoride NaF varnish and 2% chlorhexidine CHX affect these biomarkers in caries prevention among children aged 6 to 8 years. Participants are divided into four groups one receiving 5% NaF varnish, another receiving 2% CHX followed by 5% NaF varnish, a group receiving standard oral hygiene education, and a control group of caries-free children with no intervention. Applications involve drying teeth, applying varnish or solution with specific timings, and parental instructions to limit water and hard food intake for an hour post-application. Saliva samples are collected at baseline, 30 minutes after application, and after one month, with the control group sampled only once at baseline. Children and their parents provide informed consent, and samples are processed and stored under controlled conditions. Researchers measure changes in the salivary biomarkers using approved ELISA kits, with data analyzed statistically to assess differences between groups and over time. The study includes clinical assessments, oral hygiene education, and follow-up saliva collections, aiming to link biomarker changes to clinical findings over the study period ending in June 2027.

The Effects of Sodium Fluoride and Chlorhexidine Use on Salivary IL-6 and Matrix Metalloproteinase Levels in Children With Active Caries