Actively Recruiting

FEMALE
Healthy Volunteers
ID05337072

The FLORA-project: in Search for the Microbial Cause of Chronic Endometritis and the Most Appropriate Treatment to Obtain a Successful Pregnancy IVF/ICSI

Led by Universitair Ziekenhuis Brussel · Updated on 2026-05-15

1000

Participants Needed

1

Research Sites

N/A

Total Duration

On this page

AI-Summary

What this Trial Is About

This research aims to better understand chronic endometritis, an inflammation of the lining of the uterus that can affect fertility and pregnancy. It is commonly seen in about 10% of women with fertility issues. The diagnosis is challenging due to a lack of well-established classification systems, although a special scoring method using plasma cell presence is used at UZ Brussel. The study also explores the role of microorganisms in the female reproductive tract and their impact on this condition and fertility. The project involves analyzing samples from women undergoing IVF/ICSI treatment, including vaginal swabs and endometrial biopsies taken during and after hysteroscopy. These samples will be examined using advanced methods called metagenomics and culturomics to identify the microorganisms present. Researchers will compare the microbiome before and after treatment with broad-spectrum antibiotics, mainly doxycycline, to understand how these treatments affect the balance of bacteria and signs of inflammation. Participants will undergo routine diagnostic procedures such as transvaginal ultrasound, biopsies, and vaginal swabs. The study will measure the microbiome through sequencing and culture over two years, alongside the evaluation of plasma cell presence to validate the diagnostic scoring system. This process aims to improve diagnosis and guide treatment choices for chronic endometritis, supporting better fertility outcomes. Participation involves continuous monitoring of microbial changes and treatment effects throughout the study period.

CONDITIONS

Brief Title

The FLORA-project: in Search for the Microbial Cause of Chronic Endometritis and the Most Appropriate Treatment to Obtain a Successful Pregnancy IVF/ICSI

Who Can Participate

FEMALE
Healthy Volunteers

Eligibility Criteria

Eligible

You may qualify if you...

  • Women planned for IVF/ICSI treatment at Brussels IVF (UZ Brussel)
  • Women who undergo a diagnostic hysteroscopy in preparation of their treatment
Not Eligible

You will not qualify if you...

  • If an interventional instead of a diagnostic hysteroscopy is planned
  • History of chronic endometritis and use of antibiotics in the last 3 months

AI-Screening

AI-Powered Screening

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Your Study Journey

Screening

Duration - 2 to 4 weeks

Participants are screened for eligibility to participate in the trial.

1 screening and enrollment visit (in-person)

Diagnostic Evaluation

Duration - At baseline before IVF/ICSI treatment

Participants undergo diagnostic hysteroscopy with endometrial biopsies and vaginal swabs to analyze the microbiome and evaluate chronic endometritis.

1 diagnostic visit (in-person)

Long-term Monitoring

Duration - Up to 2 years

Participants are observed over time to study the microbiome changes and the presence of chronic endometritis during their IVF/ICSI treatment course.

Periodic assessments during IVF/ICSI treatment as per routine care

Trial Site Locations

Total: 1 location

1

Universitair Ziekenhuis Brussel

Brussels, Belgium, 1090

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How is the study designed?

Study Type

OBSERVATIONAL

Masking

N/A

Allocation

N/A

Model

N/A

Primary Purpose

N/A

Number of Arms

0

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