Ning De Shi

Cervical precancerous lesions, specifically Cervical Intraepithelial Neoplasia grade 2 (CIN2), pose significant risks to the health and fertility of young women. This research evaluates how the integration of Human Papillomavirus (HPV) DNA affects the progression and prognosis of CIN2 in women aged 45 and below. The study also explores the relationship between HPV integration status, vaginal flora diversity, and cervical cytology findings to improve understanding of disease outcomes. The study follows 300 women diagnosed with CIN2 confirmed by biopsy, recruiting them from multiple hospitals in China. Participants undergo comprehensive testing, including HPV viral integration tests, HPV genotyping, cervical cytology (ThinPrep tests), and vaginal secretion analysis at baseline and at 3, 6, 9, and 12 months. Colposcopy and biopsies are performed at the 6- and 12-month visits to assess lesion progression. No surgical or medication treatments are given unless the disease progresses during the 12-month observation. Participants will attend scheduled visits for sample collection and testing throughout the year-long follow-up. Researchers will perform various laboratory analyses, including HPV integration and genotyping, cervical cytology, and 16SrRNA sequencing of vaginal secretions. These assessments aim to monitor disease progression, understand HPV's role in CIN2 outcomes, and evaluate changes in vaginal flora. The study's comprehensive data collection supports a detailed evaluation of prognostic factors and disease management in young women with CIN2.

Researchers are studying the effects of normobaric oxygen (NBO) therapy on patients who have experienced an acute ischemic stroke (AIS) and are transferred for endovascular thrombectomy (EVT). The goal is to assess the safety and effectiveness of NBO in improving functional outcomes three months after stroke. Stroke is a major cause of death and disability worldwide, and while treatments like mechanical thrombectomy can improve blood flow, less than half of patients with large vessel occlusion achieve good recovery. NBO offers potential brain protection by improving oxygen delivery to affected areas and reducing damage through multiple mechanisms. Participants will receive either inhaled 100% oxygen (NBO) or best medical care without NBO. The study focuses on patients transferred for EVT who meet specific stroke severity and imaging criteria. NBO treatment is delivered through oxygen inhalation, aiming to increase oxygen availability to the brain before reperfusion. The study is conducted in a Phase 3 setting and compares outcomes between those receiving NBO and those receiving standard care. During the study, participants will be monitored for disability levels using the modified Rankin scale at 90 days and one year after treatment. Assessments include neurological exams and imaging to confirm stroke details and severity. Researchers will also track safety and functional outcomes to evaluate the impact of NBO therapy. Participants are followed for at least three months after randomization to observe recovery and potential benefits.

Researchers are investigating the clinical and molecular characteristics of cervical neuroendocrine tumors by collecting and analyzing data from 300 women diagnosed with this rare type of cervical cancer. The study aims to evaluate the prognosis and survival outcomes of these patients through detailed clinical information such as age, biopsy results, HPV genotyping, cancer staging, and follow-up data. Additionally, the study includes molecular analysis to better understand the protein features and potential prognostic markers of this cancer type. The research involves gathering paraffin tissue samples from 100 cases of cervical neuroendocrine tumors across multiple centers in China. These samples are processed to measure protein concentration and perform proteomic analyses to identify molecular characteristics that may correlate with patient outcomes. The study does not include specific treatment interventions but focuses on diagnostic and molecular data collection. Participants will be involved through data review and tissue sample analysis, with clinical follow-up information recorded to assess survival and disease progression over three years after surgery. The outcomes measured include mortality rates and disease-free survival within this timeframe. The study establishes a follow-up database to facilitate ongoing evaluation of clinical and molecular data to explore the clinical application of these findings.