Metabolic Profiling of Hematopoietic Stem Cells in Clonal Hematopoiesis (CHIP): A Prospective Observational Study

What this Trial Is About

Researchers are studying metabolic profiles of hematopoietic stem cells in patients with clonal hematopoiesis of indeterminate potential (CHIP), osteoporotic hip fractures, and hip osteoarthritis. This observational study collects blood and bone marrow samples from patients undergoing hip arthroplasty surgery to analyze gene expression and relevant CHIP mutations. The goal is to compare gene profiles of wildtype and mutant stem cells through advanced genetic and metabolic analyses. The study involves collecting blood and bone marrow cells during hip replacement surgery. These samples undergo metabolic profiling and mutation-specific single-cell genotyping using biotin-PCR with mutation-targeted primers followed by sequencing. Researchers perform combined single-cell transcriptomics and gene set enrichment analyses to evaluate metabolic pathways and gene expression differences between cell types. Participants will provide samples during surgery, with researchers analyzing the gene expression profiles using broad and targeted approaches. The main outcome is the Gene Set Enrichment Analysis (GSEA) Normalized Enrichment Score measured one day after sample processing. The study involves patients aged 65 and older undergoing hip surgery, with no interventions beyond sample collection. The research aims to better understand CHIP and its metabolic characteristics in aging and bone-related conditions.

Metabolic Profiling of Hematopoietic Stem Cells in Clonal Hematopoiesis (CHIP)