Exploring Human Spleen Function and Diseases Through Direct and Ex-vivo Perfusion Studies in Adults Undergoing Splenectomy

What this Trial Is About

Researchers are studying human splenic physiology to better understand its role in diseases affecting blood cells. The spleen helps recognize changes in circulating cells and triggers immune responses. Past studies clarified its role in malaria, and this research aims to extend that knowledge to other diseases involving the spleen. The study also evaluates substances, such as monoclonal antibodies, that might alter how the spleen perceives blood cells. The study involves adult patients scheduled for splenic interventions like spleno-pancreatectomy or total or partial splenectomy. Blood samples collected before or during surgery will be analyzed as controls. After surgery, the spleen or spleen fragments will be collected for detailed examination. When possible, the spleen is flushed with a cold perfusion medium and studied in the lab through ex-vivo perfusion to analyze how it filters altered cells. Participants will have blood and spleen samples analyzed using various techniques including cytology, cytometry, histology, and molecular biology. Samples will also be stored in a biobank for future research. Researchers monitor spleen function during infections and blood cell conditions, exploring immunological mechanisms, clearance kinetics, genetic control, and the effects of preservation on organ and cell functions. The studys duration varies with patient treatment schedules and sample processing.

Physiology and Pathologies Linked to Human Splenic Function : Direct and Ex-vivo Perfusion Explorations