Actively Recruiting
Physiology and Pathologies Linked to Human Splenic Function: Direct and Ex-vivo Perfusion Explorations
Led by Assistance Publique - Hôpitaux de Paris · Updated on 2025-09-15
100
Participants Needed
5
Research Sites
N/A
Total Duration
On this page
Sponsors
A
Assistance Publique - Hôpitaux de Paris
Lead Sponsor
I
Institut Pasteur
Collaborating Sponsor
AI-Summary
What this Trial Is About
Researchers are studying human splenic physiology to better understand its role in diseases affecting blood cells. The spleen helps recognize changes in circulating cells and triggers immune responses. Past studies clarified its role in malaria, and this research aims to extend that knowledge to other diseases involving the spleen. The study also evaluates substances, such as monoclonal antibodies, that might alter how the spleen perceives blood cells. The study involves adult patients scheduled for splenic interventions like spleno-pancreatectomy or total or partial splenectomy. Blood samples collected before or during surgery will be analyzed as controls. After surgery, the spleen or spleen fragments will be collected for detailed examination. When possible, the spleen is flushed with a cold perfusion medium and studied in the lab through ex-vivo perfusion to analyze how it filters altered cells. Participants will have blood and spleen samples analyzed using various techniques including cytology, cytometry, histology, and molecular biology. Samples will also be stored in a biobank for future research. Researchers monitor spleen function during infections and blood cell conditions, exploring immunological mechanisms, clearance kinetics, genetic control, and the effects of preservation on organ and cell functions. The study's duration varies with patient treatment schedules and sample processing.
CONDITIONS
Brief Title
Physiology and Pathologies Linked to Human Splenic Function : Direct and Ex-vivo Perfusion Explorations
Who Can Participate
Eligibility Criteria
You may qualify if you...
- Adult patients
- Patients requiring left splenopancreectomy or planned splenectomy regardless of the method or indication
You will not qualify if you...
- Patients who have refused to allow their spleen and blood to be collected for research
AI-Screening
AI-Powered Screening
Complete this quick 3-step screening to check your eligibility
Your Study Journey
Duration - 2 to 4 weeks
Participants are screened for eligibility to participate in the trial.
1 visit (in-person)
Duration - Day 0
Participants undergo planned splenic intervention such as spleno-pancreatectomy or total/partial splenectomy, during which blood and spleen samples are collected for analysis.
1 surgical visit (in-person)
Duration - Same day as surgery (Day 0)
Collected spleen and blood samples undergo ex-vivo perfusion and various analyses including cytology, immunohistochemistry, and molecular biology to explore splenic function.
Laboratory assessments on Day 0
Trial Site Locations
Total: 5 locations
1
Hôpital Beaujon
Clichy, France, 92110
Actively Recruiting
2
Hôpital Saint Antoine
Paris, France, 75012
Actively Recruiting
3
Hôpital Pitié Salpêtrière
Paris, France, 75013
Actively Recruiting
4
Hôpital Necker-Enfants Malades
Paris, France, 75015
Actively Recruiting
5
Institut Pasteur
Paris, France, 75015
Active, Not Recruiting
Research Team
P
Pierre Buffet, MD, PhD
H
Hélène Morel
How is the study designed?
Study Type
OBSERVATIONAL
Masking
N/A
Allocation
N/A
Model
N/A
Primary Purpose
N/A
Number of Arms
1
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