Actively Recruiting
A Prospective Observational Study of Cerebrospinal Fluid Mitochondrial Biomarkers Measured by Flow Cytometry in Patients With Ischemic Stroke and Alzheimer Disease Controls
Led by Capital Medical University · Updated on 2026-05-22
40
Participants Needed
1
Research Sites
13 weeks
Total Duration
On this page
AI-Summary
What this Trial Is About
This research aims to study mitochondrial biomarkers in the cerebrospinal fluid of patients with ischemic stroke and Alzheimer disease controls who undergo lumbar puncture for medical reasons. The study focuses on how mitochondrial signals in cerebrospinal fluid relate to brain injury and outcomes in ischemic stroke, comparing these markers with those in Alzheimer disease patients. It is a prospective observational study conducted at Xuanwu Hospital, Capital Medical University, enrolling patients between March and May 2026. Patients diagnosed with ischemic stroke or Alzheimer disease who have a clinical indication for lumbar puncture will have their leftover cerebrospinal fluid analyzed using flow cytometry. This analysis will measure mitochondrial content with MitoTracker, mitochondrial membrane potential with JC-1, and various membrane-associated markers to identify different cell types. The study groups include an ischemic stroke cohort and an Alzheimer disease control group, with no additional interventions beyond routine care. Participants will have residual cerebrospinal fluid collected after clinical testing for detailed biomarker analysis. Researchers will assess neurological severity using admission and discharge NIHSS scores and functional outcomes with the 90-day modified Rankin Scale in stroke patients. The study will also compare mitochondrial profiles between the two patient groups. Participation involves clinical assessments and follow-up for up to 90 days after stroke onset to evaluate these outcomes.
CONDITIONS
Brief Title
A Prospective Observational Study of Cerebrospinal Fluid Mitochondrial Biomarkers Measured by Flow Cytometry in Patients With Ischemic Stroke and Alzheimer Disease Controls
Who Can Participate
Eligibility Criteria
You may qualify if you...
- Age 18 years or older
- Diagnosed with ischemic stroke or Alzheimer disease based on standard clinical criteria
- Undergoing diagnostic lumbar puncture for clinical reasons
- Residual cerebrospinal fluid available after clinical testing
- Able to provide written informed consent or have a legal representative provide consent
- For ischemic stroke patients, baseline neurological assessment available and planned 90-day follow-up for functional outcome
You will not qualify if you...
- Lumbar puncture performed only for research purposes
- Insufficient cerebrospinal fluid for analysis
- Cerebrospinal fluid sample is grossly bloody or severely contaminated
- Known central nervous system infection, malignant meningitis, or other inflammatory or neoplastic conditions affecting measurements
- Unable to give informed consent
- Missing key clinical outcome data for ischemic stroke patients
- Any condition deemed unsuitable by the investigator for study participation
AI-Screening
AI-Powered Screening
Complete this quick 3-step screening to check your eligibility
Your Study Journey
Duration - 2 to 4 weeks
Participants are screened for eligibility to participate in the trial.
1 visit (in-person)
Duration - At baseline
Participants undergo diagnostic lumbar puncture as part of routine clinical evaluation, and residual cerebrospinal fluid is collected for research flow cytometry analysis to assess mitochondrial content and related markers.
1 visit (in-person) during routine clinical care
Duration - Up to 90 days
Participants are monitored for clinical outcomes including neurological assessments and functional status up to 90 days after stroke onset or hospital admission.
Follow-up visits as part of routine care up to 90 days
Trial Site Locations
Total: 1 location
1
Xuanwu Hospital
Beijing, None Selected, China, 100053
Actively Recruiting
Research Team
X
Xi Chen, MD/PhD
How is the study designed?
Study Type
OBSERVATIONAL
Masking
N/A
Allocation
N/A
Model
N/A
Primary Purpose
N/A
Number of Arms
2
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