Actively Recruiting

Age: 18Years +
All Genders
ID06791889

Blood DdPCR for Early Identification and Dynamic Surveillance of Pathogenic Bacteria in ICU Septic Patients: a Single-centre, Prospective, Observational Study

Led by Union Hospital, Tongji Medical College, Huazhong University of Science and Technology · Updated on 2025-03-17

60

Participants Needed

1

Research Sites

N/A

Total Duration

On this page

AI-Summary

What this Trial Is About

Researchers are investigating how droplet digital PCR (ddPCR) can help identify harmful bacteria early in patients admitted to the intensive care unit (ICU) with sepsis. This observational study aims to track the presence and amount of bacteria in the blood over time and understand how these bacteria relate to patients' clinical conditions and outcomes. The study is conducted at a single center and focuses on critically ill ICU patients with their first sepsis diagnosis. Patients in this study will have their blood tested at various points from admission up to 28 to 30 days or until they leave the ICU. The ddPCR method will be used to monitor the amount of bacterial genetic material in their blood dynamically. This ongoing monitoring helps researchers observe changes in bacterial presence during the critical period of sepsis treatment. Participants will undergo repeated blood sampling at specified intervals: days 0, 2-3, 4-5, 6-7, 8-9, 10-12, 13-15, 20-22, and 28-30 after ICU admission. Researchers will measure bacterial nucleic acid loads at these times and track survival status at 28 days after the initial sepsis diagnosis. This monitoring helps assess the relationship between bacterial levels and patient outcomes throughout the observation period.

CONDITIONS

Brief Title

Blood DdPCR for Early Identification and Dynamic Surveillance of Pathogenic Bacteria in ICU Septic Patients: a Single-centre, Prospective, Observational Study

Who Can Participate

Age: 18Years +
All Genders

Eligibility Criteria

Eligible

You may qualify if you...

  • Patients with first diagnosis of sepsis after ICU admission
Not Eligible

You will not qualify if you...

  • Age under 18 years
  • Do not meet Sepsis 3.0 diagnostic criteria
  • Refusal to sign informed consent

AI-Screening

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Your Study Journey

Screening

Duration - 2 to 4 weeks

Participants are screened for eligibility to participate in the trial.

1 visit (in-person)

Monitoring

Duration - Up to 28 to 30 days or until ICU discharge

Participants are dynamically monitored for nucleic acid loads of pathogenic bacteria in the blood at different time points until day 28-30 or discharge from the ICU.

Multiple blood sample collections at scheduled intervals during ICU stay

Trial Site Locations

Total: 1 location

1

Department of Critical Care Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology

Wuhan, China

Actively Recruiting

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Research Team

Y

You Shang, prof.

X

Xiaobo Yang, prof.

How is the study designed?

Study Type

OBSERVATIONAL

Masking

N/A

Allocation

N/A

Model

N/A

Primary Purpose

N/A

Number of Arms

1

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