Measuring Nitrous Oxide Levels in Blood, Breath, and Urine After Different Types of Exposure in Adults Aged 18 to 65

What This Trial Is About

Researchers are studying the levels of nitrous oxide in the blood, exhaled air, and urine after different types of exposures among adults aged 18 to 65. The study compares three specific exposures inhaling nitrous oxide released in a car cabin, consuming whipped cream containing nitrous oxide as a propellant, and exposure through work as a midwife in a delivery room. This investigation aims to understand how nitrous oxide concentrations change over time following these exposures. Participants experience one of the following exposure methods passive inhalation of nitrous oxide released from three or four cartridges in a closed car cabin, consumption of 100 grams of whipped cream containing nitrous oxide, or environmental exposure from midwives working in delivery rooms. After each exposure, concentrations of nitrous oxide are measured at various time points to track how long the gas stays in the body. During the study, nitrous oxide levels are measured in blood and exhaled air for up to one hour after exposure and in urine for up to two hours using techniques like gas chromatography, mass spectrometry, and specialized sensors. Participants are monitored closely, and the primary outcome is the detection time of nitrous oxide in the blood from the end of exposure to one hour afterward. The total participation period covers the exposure and subsequent monitoring times as described.

Concentrations of Nitrous Oxide in Blood, Exhaled Air and Urine After Various Types of Exposure.