Aging and the environment affect gamete and embryo potential: can we intervene?
David R Meldrum, Robert F Casper, Antonio Diez-Juan...
https://pubmed.ncbi.nlm.nih.gov/26812244Actively Recruiting
Phase Not Applicable
Age: 18Years - 43Years
All Genders
Healthy Volunteers
ID06261671
Antioxidants-enriched Single-step vs Global Total Single-step Embryo Culture Media Effect on Blastocyst Euploidy Rates
Led by ART Fertility Clinics LLC · Updated on 2025-02-21
500
Participants Needed
1
Research Sites
N/A
Total Duration
On this page
AI-Summary
What this Trial Is About
Researchers are investigating the impact of antioxidant-enriched culture media on embryo development during in vitro fertilization (IVF). The study focuses on how reactive oxidative species (ROS) generated in the artificial IVF environment may affect gametes and embryos, potentially influencing embryo quality and development. This prospective randomized trial uses sibling oocytes to assess how antioxidant supplements in culture media might improve embryo ploidy rates, fertilization, and blastocyst development. Participants' embryos will be cultured using two different media: one enriched with antioxidants (Gx) and the other using the Global total one step media (GT). Both groups will be cultured continuously without media refreshment on day 3, but the media will be refreshed on day 5. The study compares the effects of these two culture media on embryo development through IVF procedures. During the study, embryos will be biopsied on days 5, 6, or 7 to assess blastocyst ploidy by examining trophectoderm cells. Researchers will monitor outcomes such as normal or abnormal ploidy, inconclusive results, and mosaicism levels. Secondary outcomes include the quality of blastocysts at biopsy and usable blastocyst rates per group. The study will last up to one year, with participants undergoing IVF cycles involving intracytoplasmic sperm injection (ICSI) and having multiple assessments of embryo development and quality.
CONDITIONS
Brief Title
Effect of Antioxydant-enriched Media on Blastocyst Euploidy Rates.
Who Can Participate
Age: 18Years - 43Years
All Genders
Healthy Volunteers
Eligibility Criteria
You may qualify if you...
- Patients undergoing assisted reproductive technology cycles when ICSI is indicated
- Patients undergoing IVF included if enough oocytes are available for ICSI randomization; IVF oocytes not used
- Maternal age between 18 and 43 years old
- PGT-A cycles with trophectoderm biopsies on day 5, 6, or 7
- Patients expected to have more than 6 cumulus-oocyte complexes (COCs) for ICSI
- Body mass index less than 35
- Use of fresh or frozen ejaculated sperm
You will not qualify if you...
- PGT-M cycles
- Use of fresh or frozen testicular sperm
AI-Screening
AI-Powered Screening
Complete this quick 3-step screening to check your eligibility
1
2
3
Your Study Journey
Screening
Duration - 2 to 4 weeks
Participants are screened for eligibility to participate in the trial.
1 visit (in-person)
Treatment
Duration - Up to 7 days of embryo development
Participants undergo IVF with oocytes cultured in either antioxidants-enriched or standard culture media to assess embryo development and euploidy rates.
1 IVF cycle with embryo culture and biopsy on day 5, 6, or 7
Trial Site Locations
Total: 1 location
1
ART Fertility Clinics LLC
Abu Dhabi, Abu Dhabi Emirate, United Arab Emirates, 60202
Actively Recruiting
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Research Team
D
Daniela Nogueira
J
Jonalyn DV Edades
How is the study designed?
Study Type
INTERVENTIONAL
Masking
NONE
Allocation
RANDOMIZED
Model
PARALLEL
Primary Purpose
TREATMENT
Number of Arms
2
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Published Research Related To This Trial
Redox Biology of Human Cumulus Cells: Basic Concepts, Impact on Oocyte Quality, and Potential Clinical Use.
Lucia von Mengden, Fabio Klamt, Johan Smitz
https://pubmed.ncbi.nlm.nih.gov/31861967Oxidative stress and antioxidants: exposure and impact on female fertility.
Elizabeth H Ruder, Terryl J Hartman, Jeffrey Blumberg...
https://pubmed.ncbi.nlm.nih.gov/18535004International Committee for Monitoring Assisted Reproductive Technologies world report: assisted reproductive technology, 2014†.
Georgina M Chambers, Silke Dyer, Fernando Zegers-Hochschild...
https://pubmed.ncbi.nlm.nih.gov/34601605DNA damage by oxygen-derived species. Its mechanism and measurement in mammalian systems.
B Halliwell, O I Aruoma
https://pubmed.ncbi.nlm.nih.gov/1849843Cellular reducing equivalents and oxidative stress.
J P Kehrer, L G Lund
https://pubmed.ncbi.nlm.nih.gov/7959167Impact of oxidative stress on male and female germ cells: implications for fertility.
R John Aitken
https://pubmed.ncbi.nlm.nih.gov/31846434Impact of oxidative stress on female fertility.
Elizabeth H Ruder, Terryl J Hartman, Marlene B Goldman
https://pubmed.ncbi.nlm.nih.gov/19469044Role of sperm chromatin abnormalities and DNA damage in male infertility.
A Agarwal, Tamer M Said
https://pubmed.ncbi.nlm.nih.gov/12926527Antioxidant supplementation overcomes the deleterious effects of maternal restraint stress-induced oxidative stress on mouse oocytes.
Hua-Yu Lian, Yan Gao, Guang-Zhong Jiao...
https://pubmed.ncbi.nlm.nih.gov/24043846