Study Comparing Antioxidant-Enriched and Standard Culture Media on Embryo Chromosome Health in IVF Patients Aged 18 to 43

What this Trial Is About

Researchers are investigating the impact of antioxidant-enriched culture media on embryo development during in vitro fertilization IVF. The study focuses on how reactive oxidative species ROS generated in the artificial IVF environment may affect gametes and embryos, potentially influencing embryo quality and development. This prospective randomized trial uses sibling oocytes to assess how antioxidant supplements in culture media might improve embryo ploidy rates, fertilization, and blastocyst development. Participants embryos will be cultured using two different media one enriched with antioxidants Gx and the other using the Global total one step media GT. Both groups will be cultured continuously without media refreshment on day 3, but the media will be refreshed on day 5. The study compares the effects of these two culture media on embryo development through IVF procedures. During the study, embryos will be biopsied on days 5, 6, or 7 to assess blastocyst ploidy by examining trophectoderm cells. Researchers will monitor outcomes such as normal or abnormal ploidy, inconclusive results, and mosaicism levels. Secondary outcomes include the quality of blastocysts at biopsy and usable blastocyst rates per group. The study will last up to one year, with participants undergoing IVF cycles involving intracytoplasmic sperm injection ICSI and having multiple assessments of embryo development and quality.

Effect of Antioxydant-enriched Media on Blastocyst Euploidy Rates.