Antibacterial Effects of 940nm Diode Laser, Apple Cider Vinegar, and Gymnema Sylvestre in Adults with Type 2 Diabetes Undergoing Root Canal Treatment

What this Trial Is About

This research evaluates the antibacterial effects of different disinfection methods during root canal treatment in patients with type 2 diabetes and apical periodontitis. The study is a randomized controlled clinical trial conducted over one year, aiming to detect Peptostreptococcus species in infected root canals. The trial compares the use of a 940 nm diode laser, apple cider vinegar, Gymnema Sylvestre, and combinations of these against standard saline irrigation in diabetic patients aged 25 to 60 years. Participants undergo standard root canal treatment with aseptic isolation and access cavity preparation. Working length is determined by electronic apex locator and radiography. Canals are instrumented and irrigated using 5% sodium hypochlorite and 17% EDTA. They are then randomly assigned to one of six groups receiving either saline, apple cider vinegar, diode laser, Gymnema Sylvestre solution, or combinations of diode laser with apple cider vinegar or Gymnema Sylvestre. Post-disinfection microbial samples are collected for PCR analysis. Patients are followed up at 7, 15, 30, 45, and 60 days after treatment. Clinical outcomes are assessed using periapical radiographs and the Periapical Index PAI. Pain intensity is measured by the Visual Analog Scale VAS. Microbial presence of Peptostreptococcus species is monitored over one year using PCR. The study also tracks pain levels and treatment effects through repeated assessments during the follow-up period.

Effect of Diode Laser, Gudmar and Vinegar in Diabetic Patients During Root Canal Treatment