Studying Blood miRNAs to Find New Ways to Diagnose Adenomyosis in Women Aged 18 and Older

What this Trial Is About

This research aims to identify differences in circulating microRNAs miRNAs in the blood of women with adenomyosis to develop new, noninvasive diagnostic methods. Building on previous findings that miRNAs are abnormally expressed in conditions like endometriosis, this study will compare miRNA expression between patients with adenomyosis and healthy subjects to find potential diagnostic biomarkers. Blood samples will be collected from 50 pre-menopausal women undergoing gynecological surgery, including 25 diagnosed with adenomyosis and 25 without endometrial-related diseases. A single 10 ml blood sample will be taken on the day of surgery, processed to isolate miRNAs using specialized kits, and analyzed using commercial arrays and RT-qPCR to measure expression of around 1000 miRNAs. The study will also investigate the biological functions affected by differentially expressed miRNAs. Participants will have their blood drawn during surgery for sample analysis. Researchers will examine miRNA levels as diagnostic biomarkers throughout the study, which lasts approximately 6.5 years. Samples will be stored carefully, and data will be analyzed to identify miRNAs distinguishing adenomyosis patients from healthy individuals. The findings could support the development of new diagnostic tools or treatments for adenomyosis.

New Perspectives in Adenomyosis Pathogenesis With Epigenetic Analysis and miRNAs