Study of Metabolic Flexibility in Adults Aged 45 to 65 Comparing Effects of Cheese from Extensive and Intensive Farming

What this Trial Is About

Researchers are evaluating how consuming meat and dairy products from different farming methods affects metabolic health in healthy adults aged 45 to 65 years. This age range is linked to changes in metabolism and body fat. The study focuses on key markers like the gene expression of the transcription factor PPAR-b1 in white blood cells, fatty acid metabolism, and the endocannabinoid system. It also examines metabolic flexibility through exercise testing and measures body composition. Participants will take part in a double-blind, randomized, controlled crossover trial involving two types of pecorino cheese kenterbas from extensive farming and industrial pecorino from intensive farming. Each participant will consume 350 grams per week of one cheese type for 4 weeks, followed by an 8-week washout period without cheese consumption. Then, they will switch to the other cheese type for another 4 weeks. Blood samples will be collected at the end of each period to analyze metabolic changes. Throughout the study, participants will maintain their usual lifestyle and diet. Researchers will gather medical history, anthropometric measurements, and blood samples for detailed analysis. Body composition will be assessed by bioimpedance and waist measurements, while metabolic flexibility will be tested using indirect calorimetry during exercise. The main outcome is the change in plasma levels of DHA and acylethanolamides, with secondary outcomes assessing respiratory quotient changes. The total study duration includes treatment, washout, and crossover phases.

Assessment Metabolic Flexibility in Middle-aged Individuals: The Nutritional Impact of Cheese Consumption