Comparing Two Root Canal Cleaning Methods for Reducing Bacteria in Teeth with Apical Periodontitis Using Two Microbiological Tests

What this Trial Is About

Researchers are studying two supplementary antimicrobial methods to disinfect teeth affected by apical periodontitis. The study compares the effectiveness of laser activated irrigation using the Er,CrYSGG laser and ultrasonic activation with a piezoelectric ultrasonic device and Irrisafe tip. This randomized clinical trial aims to measure how well these treatments reduce bacteria in the root canal system. Participants receive one of two disinfection protocols either the laser activated irrigation with a radial-firing tip or ultrasonic activation using the piezoelectric device. The study also uses two microbiological methods to assess bacteria presence before and after treatment ATP level analysis with the Endocator device and quantitative PCR for bacterial DNA quantification. These methods help evaluate bacterial reduction immediately after irrigation and at the end of the study. During the study, participants undergo treatment of their molar teeth that require endodontic care and meet specific criteria. Researchers measure bacterial levels through rapid ATP testing and qPCR analysis. The main outcome is the reduction of bacteria in the root canal after the irrigation procedures. The trial includes follow-up assessments to ensure accurate microbiological evaluation, and participation lasts until all samples have been collected and analyzed.

Comparison of Root Canal Desinfection Protocols