Studying the Impact of 84-Hour Fasting on Fat Burning, Mitochondrial Function, and Tissue Health in Healthy Young Adults with Normal Weight or Obesity

What this Trial Is About

Researchers are studying how 84 hours of fasting affects peak fat burning, mitochondrial function, and tissue health in young healthy men and women with normal weight or obesity. The study includes four groups of 10 participants each young normal weight men and women, and young obese men and women. This research aims to understand how fasting influences fat metabolism and protein changes in fat tissue. Participants will complete dietary and exercise records before the study. On two separate test daysbefore and after fastingthey will undergo body composition scans, blood pressure and energy use measurements, blood sampling, and tissue biopsies. Participants will also perform exercise tests to measure fat burning and strength. During the 84-hour fasting period, participants will communicate daily with researchers and perform light exercise while receiving electrolyte supplements. Throughout the study, participants will be monitored with physiological tests, blood samples, tissue biopsies, and exercise assessments. Researchers will measure fastings impact on protein changes in fat tissue and peak fat oxidation. The total study participation includes the initial assessments, fasting period, and follow-up testing, with ongoing contact to ensure participant well-being.

The Effect of Fasting on Peak Fat Oxidation, Mitochondrial Coupling and Extracellular Matrix