Assessing Metabolic Health With Stable Isotope Tracer Techniques in Adults With Obesity

What this Trial Is About

Researchers are investigating metabolic health in adults with obesity by studying protein and glucose metabolism using stable isotope tracer techniques. This interventional trial involves adults aged 19 to 59 who have obesity, defined by specific body mass index and waist circumference criteria. The study aims to better understand how the body processes protein and glucose in this population. Participants will undergo two study procedures in a crossover design. In the first, they will consume six hourly drinks containing stable isotopes of phenylalanine and NaH13CO2, designed to provide half of their daily protein intake along with extra energy from carbohydrates and fats. Blood, breath, urine, and muscle biopsy samples will be collected to analyze protein metabolism over six hours. In the second procedure, participants will consume a single 75g glucose drink labeled with stable isotopes, with blood and breath samples collected over three hours to assess glucose metabolism. Participation involves an initial virtual consent and screening with questionnaires assessing health, physical activity, and eligibility. During study visits, participants provide urine, blood, breath, and muscle biopsy samples. The muscle biopsy involves a small tissue sample taken under local anesthesia. Researchers will measure protein efficiency using the IAAO method and oral glucose tolerance with dual tracer techniques. Safety and recovery from biopsies will be monitored, and total blood collected is about 88 mL. The study runs until the end of 2026 with follow-up contacts to assess healing.

Stable Isotopes- Adults With Obesity