Comparing Inflammatory Biomarker Changes in Dried Blood Spot and Venous Blood Samples Following Controlled Stressors

What this Trial Is About

Researchers are evaluating whether dried blood spot DBS samples can measure changes in inflammatory biomarkers as accurately as traditional venous blood samples. The study focuses on comparing these two methods following controlled physiological stressors, such as vaccination or planned events that can temporarily raise inflammation. This research aims to determine if DBS can serve as a reliable, less invasive alternative for measuring inflammation in both acute and chronic settings. The study involves collecting both venous blood samples and DBS samples from participants. Venous samples will be processed and stored under controlled conditions, while DBS samples will be collected via finger prick onto filter paper, dried, and stored with desiccants following validated guidelines. The researchers will use a comprehensive panel that measures 48 inflammatory biomarkers to compare the results from both sample types after exposure to a physiological stressor. Participants will undergo sampling from enrollment through three days after their exposure to the stressor. Researchers will assess the agreement between inflammatory biomarker concentrations measured in DBS and venous samples. The study includes monitoring biomarker changes over this period, which helps evaluate the quality, sensitivity, and reproducibility of DBS compared to venous blood sampling. The total participation duration spans from enrollment until three days post-stressor exposure.

Analysis of Inflammatory Biomarker Changes in Dry Blood Spot Versus Venous Blood Samples