Study of Skeletal Muscle Protein Changes During Fatigue and Aging in Healthy Adults Aged 18-35 and 65-80

What this Trial Is About

Researchers are investigating the changes in proteins within skeletal muscle that contribute to the loss of muscle size and function associated with aging. The study focuses on understanding the molecular mechanisms behind age-related muscle weakening, aiming to improve physical rehabilitation for older adults at risk of mobility problems and disability. This research explores how modifications to specific muscle proteins may lead to an aging phenotype in skeletal muscle, potentially revealing new ways to enhance physical function in frail older adults. Volunteers will perform one-legged knee extension exercises until fatigue while seated on a specialized ergometer. Following the exercise, participants will undergo muscle biopsies from both legs in the thigh area. The exercise involves repeated maximum voluntary contractions of the dominant leg against resistance until muscle fatigue is reached, which typically takes between 1 and 3 minutes. Participants will be involved in muscle function assessments including measurements of muscle fiber force, tension, velocity, and power within three weeks after the biopsies. The primary outcome is the phosphorylation level of myosin binding protein C measured one week post-biopsy. The study includes physical activity monitoring and requires participants to complete all aspects including the exercise and biopsy procedures. The total participation period and safety follow-up extend up to three weeks after intervention.

C-Protein in Fatigue and Aging