Effect of Different Irrigation Activating Techniques on Irrigant Penetration Depth and Microbial Reduction in Root Canals

What this Trial Is About

This research aims to evaluate how different irrigation activation techniques affect the depth of irrigant penetration and the reduction of microbes in root canals. It focuses on root canal treatment challenges, such as complex root canal anatomy and microbial infection, which make complete cleaning difficult. The study investigates methods to improve the cleaning effectiveness beyond mechanical instrumentation by activating chemical irrigants to reach the canal's entire surface, especially the apical third. The study compares three irrigation activation methods: manual dynamic agitation using matching gutta-percha master cones, the EasyClean system operated at high rotation speeds, and the XP-endo Finisher file used with continuous rotation and specific movements. Each method is applied after standard root canal preparation. The irrigant penetration is assessed using Iohexol contrast media visible on dental radiographs, and microbial samples are collected before and after treatment to evaluate bacterial reduction. Participants will undergo root canal treatment with microbial samples taken at multiple stages to measure bacterial presence. Digital radiographs will be used to measure how far the irrigant penetrates in the canal. Researchers will measure irrigant penetration depth on the same day and bacterial reduction 24-28 hours after culturing. This study includes monitoring safety and evaluating the effectiveness of activation techniques in enhancing irrigation and reducing microbes during root canal treatment.

Irrigation Activating Techniques on Irrigant Penetration Depth and Microbial Reduction